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14. Extracting, Isolating, and Visualizi... > Procedure III-5-1: Extracting and Vi...

Procedure III-5-1: Extracting and Visualizing DNA

  1. If you have not already done so, put on your goggles, gloves, and protective clothing.

  2. Transfer five drops of methylene blue stain and five drops of eosin Y stain to one well of the reaction plate. Use a toothpick to mix the solutions thoroughly, and place the reaction plate aside for later use.

  3. Make up 50 mL of normal saline by dissolving 0.45 g (one slightly rounded spatula spoon) of table salt in 50 mL of distilled water in a 50 mL centrifuge tube, and place the tube aside for later use.

  4. Transfer about 40 mL of isopropanol to a second 50 mL centrifuge tube. Cap the tube and place it in the freezer to cool.

    Note

    99% isopropanol yields the best results, but 91% or even 70% isopropanol is usable. You can also substitute ethanol for isopropanol.


  

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