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6. Staining > Procedure I-3-1: Simple Staining

Procedure I-3-1: Simple Staining

  1. Transfer one drop of distilled water to the center of a slide.

  2. Use the flat end of a toothpick to scrape (gently) the inside of your cheek.

  3. Immerse the end of the toothpick in the drop of water and stir to transfer the epithelial cells to the water. Position a coverslip over the specimen. If there are bubbles under the coverslip, press gently on the coverslip with your forceps to force the bubbles out from under the coverslip.

  4. Position the slide in the stage clips or mechanical stage, rotate the 4X objective into position, turn on the illuminator, adjust the illumination, and focus on the slide.

  5. Center an epithelial cell or a group of cells in the field of view and then rotate the 10X objective into position. Again center a cell or group of cells, and then rotate the 400X objective into position and focus critically.


  

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