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Procedure III-1-2: Effect of Concentrati... > Populating the Reaction Plate
Populating the Reaction Plate
The first step is to populate the reaction plate with various dilutions of the four solutions whose pH we’ll be testing. We’ll use a procedure called serial dilution to produce ten-fold dilutions from each well to the next. For example, well A1 will contain 1.0 M acetic acid, well B1 0.1 M acetic acid, well C1 0.01 M acetic acid, and so on.
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Fill the clean 50 mL beaker nearly full with distilled water.
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Using a clean pipette, transfer nine drops of distilled water to each of wells B1 through H1, B3 through H3, B5 through H5, and B7 through H7 of the reaction plate.
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Use a clean pipette to transfer a few drops of 1 M acetic acid to well A1 and one drop of 1 M acetic acid to well B1. Return any acid still in the pipette to the labeled container.