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Procedure III-3-2: Investigating Enzyme ... > Test the Activity of Peroxidase Solu...

Test the Activity of Peroxidase Solutions

  1. If you have not already done so, put on your goggles, gloves, and protective clothing.

  2. Label six test tubes, A through F, transfer about 5 mL of the freshly prepared hydrogen peroxide solution to each tube, and place them in the rack.

  3. Transfer one drop of the unmodified peroxidase solution from centrifuge tube A to test tube A, and return any unused peroxidase solution to the centrifuge tube. Swirl the test tube, and observe the formation of bubbles. Record your observations in your lab notebook.

  4. Using a clean (or well-rinsed pipette), transfer one drop of the modified peroxidase solution from centrifuge tube B to test tube B, return any unused peroxidase solution to the centrifuge tube, swirl the test tube, and observe the formation of bubbles. Record your observations in your lab notebook.


  

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