Aseptic technique

All microbiological procedures require aseptic technique, which refers to all the activities microbiologists perform to keep unwanted microbes out of pure cultures or sterile items. Aseptic means free from germs, and sepsis is a medical term for the presence of germs.

Media, glassware, and anything else that comes in contact with live cultures must be sterilized in an autoclave. This piece of equipment treats liquids and solids with pressurized steam to kill all microbes. Items that have been sterilized and covered can be stored indefinitely.