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If you’re going through the trouble of putting your camera on a tripod (and you absolutely should), you can still get a “less-than-tack-sharp” photo from the vibration that happens when you push the shutter button. That’s why, when shooting macro, you should either use a shutter release cable (a cord that attaches to your camera that lets you take a shot without touching the shutter button on the camera itself) or use your camera’s self-timer, which takes the shot for you about 10 seconds after you press the shutter button, so any vibration caused by your pressing the shutter button will be long gone.